Monday 23 May 2011, at 16h30 in Celestijnenlaan 200A Auditorium 00.225
Peptide Identification using Mass Spectrometry Data
By Eduardo De Paula Costa (PhD student DTAI)
Peptides are short proteins that play a key role in many physiological processes such as blood pressure regulation, water balance and glucose metabolism. Hence, peptide identification is an important step in understanding how these processes work. Currently, one of the most important techniques for peptide identification is Mass spectrometry (MS), an analytical technique used for measuring the molecular mass of a biological sample. MS produces various types of data. The most common data representation is the mass spectrum. This spectrum is a plot containing the chemical analysis of a molecule (a peptide, for example) and it is used for the molecule identification. As spectrum identification can be a complex and time consuming task, many computational methods and tools have been proposed to assist researches in this task. In this presentation, I will introduce the main aspects involving peptide identification using mass spectrometry data and I will discuss the main computational approaches currently used to interpret mass spectra. More specifically, I will present a method that we are currently developing, which extends the data base search approach for peptide identification by considering additional genomic information during the spectrum identification task.